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Cu,Zn‐superoxide dismutase of Saccharomyces cerevisiae is required for resistance to hyperosmosis
Author(s) -
Garay-Arroyo Adriana,
Lledı́as Fernando,
Hansberg Wilhelm,
Covarrubias Alejandra A
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00199-6
Subject(s) - saccharomyces cerevisiae , superoxide dismutase , chemistry , osmotic shock , sod1 , antioxidant , yeast , biochemistry , superoxide , scavenger , osmotic concentration , mutant , microbiology and biotechnology , enzyme , biology , gene
Here we analyzed the role of the antioxidant response in Saccharomyces cerevisiae adaptation to hyperosmotic stress. We show that Cu,Zn‐superoxide dismutase (SOD1) plays a fundamental role in this adaptation process since under hyperosmosis SOD1 mutants lead to high protein oxidation levels and show a sensitive phenotype, which is reversed by the addition of N ‐acetylcysteine to the medium. Pretreatment with MnCl 2 , a superoxide scavenger, improves the survival of the sod1 strain upon hyperosmosis. Additionally, we show that upon hyperosmotic shock there is a small and transient increase in SOD1 transcript levels, regulated by the protein kinase A‐cAMP and SKN7 pathways.