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Potato tuber phospholipids contain colneleic acid in the 2‐position
Author(s) -
Fauconnier Marie-Laure,
Williams Todd D.,
Marlier Michel,
Welti Ruth
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00171-6
Subject(s) - phosphatidylethanolamine , phosphatidylcholine , phosphatidylinositol , phospholipid , biochemistry , chemistry , lipase , phospholipase c , phospholipase , linoleic acid , hydrolysis , phospholipase d , enzyme , chromatography , fatty acid , kinase , membrane
Colneleic acid (9‐[1′( E ),3′( Z )‐nonadienyloxy]‐8( E )‐nonenoic acid) is produced from linoleic acid by the sequential action of 9‐lipoxygenase and divinyl ether synthase. We demonstrate that a small fraction of the colneleic acid in potato tubers is esterified in phospholipids. This colneleic acid was released by chemical hydrolysis and a phospholipase A 2 , but not by a lipase with 1‐acyl specificity. Phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol contain molecular species with nominal masses consistent with identification as palmitoyl,colneleoyl species. Exact mass analysis of its fragments confirmed the identity of palmitoyl,colneloyl phosphatidylinositol. To our knowledge, this work represents the first identification of a colneleoyl phospholipid.