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Developmental regulation of β‐1,3‐galactosyltransferase‐1 gene expression in mouse brain
Author(s) -
Zhu Dan,
Shen Aiguo,
Wang Ying,
Gu Xiaosong,
Gu Jianxin
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00168-6
Subject(s) - galactosyltransferase , cerebellum , hippocampus , in situ hybridization , gene expression , biology , northern blot , cerebral cortex , western blot , immunohistochemistry , cortex (anatomy) , microbiology and biotechnology , gene , cerebellar cortex , endocrinology , medicine , enzyme , biochemistry , neuroscience , immunology
β‐1,3‐galactosyltransferase‐1 (β3GalT‐1) is the key enzyme to form the type 1 chain structure. Northern blot analysis indicated that β3GalT‐1 was expressed predominantly in the brain. In the present study, it was revealed that the gene expression of β3GalT‐1 in mouse brain was developmentally decreased. High expression levels of β3GalT‐1 were found in cerebral cortex and hippocampus in both newborn and adult mice, while in cerebellum, the expression levels decreased markedly during development. In situ hybridization revealed that the absence of expression in cerebellar granual cell layers contributed to the main loss of β3GalT‐1 expression in adult mouse cerebellum. Moreover, the decreased levels of β3GalT‐1could affect the synthesis of type 1 chain oligosaccharides, as revealed by immunohistochemistry analysis.