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In vivo studies of liver‐type fatty acid binding protein (L‐FABP) gene expression in liver of transgenic zebrafish ( Danio rerio )
Author(s) -
Her Guor Mour,
Chiang Chia-Chang,
Chen Wen-Ya,
Wu Jen-Leih
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00157-1
Subject(s) - zebrafish , biology , danio , transgene , green fluorescent protein , microbiology and biotechnology , gene , gene expression , liver regeneration , genetically modified mouse , biochemistry , regeneration (biology)
Mammalian liver fatty acid binding protein (L‐FABP) is a small cytosolic protein in various tissues including liver, small intestine and kidney and is thought to play a crucial role in intracellular fatty acid trafficking and metabolism. To better understand its tissue‐specific regulation during zebrafish hepatogenesis, we isolated 5′‐flanking sequences of the zebrafish L‐FABP gene and used a green fluorescent protein (GFP) transgenic strategy to generate liver‐specific transgenic zebrafish. The 2.8‐kb 5′‐flanking sequence of zebrafish L‐FABP gene was sufficient to direct GFP expression in liver primordia, first observed in 2 dpf embryos and then continuously to the adult stage. This pattern of transgenic expression is consistent with the expression pattern of the endogenous gene. F2 inheritance rates of 42–51% in all the seven transgenic lines were consistent with the ratio of Mendelian segregation. Further, hhex and zXbp‐1 morphants displayed a visible liver defect, which suggests that it is possible to establish an in vivo system for screening genes required for liver development.