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A novel protein phosphatase 2C family member (PP2Cζ) is able to associate with ubiquitin conjugating enzyme 9 1
Author(s) -
Kashiwaba Mitsuhiro,
Katsura Koji,
Ohnishi Motoko,
Sasaki Mutsuo,
Tanaka Hiromitsu,
Nishimune Yoshitake,
Kobayashi Takayasu,
Tamura Shinri
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00153-4
Subject(s) - ubiquitin , phosphatase , ubiquitin conjugating enzyme , enzyme , amino acid , microbiology and biotechnology , western blot , peptide sequence , biochemistry , acid phosphatase , chemistry , biology , ubiquitin ligase , gene
In this study we have cloned a novel member of mouse protein phosphatase 2C family, PP2Cζ, which is composed of 507 amino acids and has a unique N‐terminal region. The overall similarity of the amino acid sequence between PP2Cζ and PP2Cα was 22%. On Northern blot analysis PP2Cζ was found to be expressed specifically in the testicular germ cells. PP2Cζ expressed in COS7 cells was able to associate with ubiquitin conjugating enzyme 9 (UBC9) and the association was enhanced by co‐expression of small ubiquitin‐related modifier‐1 (SUMO‐1), suggesting that PP2Cζ exhibits its specific role through its SUMO‐induced recruitment to UBC9.