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Osmomechanical stress selectively regulates translocation of protein kinase C isoforms
Author(s) -
Liu X.,
Zhang M.I.N.,
Peterson L.B.,
O'Neil R.G.
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00150-9
Subject(s) - protein kinase c , cytosol , gene isoform , microbiology and biotechnology , signal transduction , chromosomal translocation , biology , chemistry , biochemistry , enzyme , gene
Osmomechanical stress, resulting in cell swelling and activation/regulation of numerous cellular processes, may play a critical role in cell signaling by selectively regulating translocation of protein kinase C (PKC) isoforms from cytosol to membrane compartments. Western blotting of renal epithelial cell fractions demonstrated the expression of five PKC isoforms. Three of these isoforms (PKCα, PKCϵ, PKCζ) translocated to the membrane fraction upon exposure of cells to osmomechanical stress (hypotonic medium). Immunohistochemical staining of cells using isoform‐specific antibodies further demonstrated translocation of the phorbol ester‐sensitive isoforms, PKCα and PKCϵ, to both the plasma membrane and perinuclear sites, reflecting potential initial steps in regulation of specific effector pathways. Indeed, selective inhibition of PKCs indicates a potential role for PKCα in modulating a calcium influx channel. It is concluded that osmomechanical stress induces selective translocation of specific PKC isoforms, demonstrating a key role of osmomechanical stress in selectively regulating PKC‐dependent signaling pathways.