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Involvement of caveolin‐2 in caveolar biogenesis in MDCK cells
Author(s) -
Lahtinen Ulla,
Honsho Masanori,
Parton Robert G.,
Simons Kai,
Verkade Paul
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00135-2
Subject(s) - caveolae , caveolin 1 , microbiology and biotechnology , caveolin , biogenesis , intracellular , chemistry , membrane , biology , biochemistry , signal transduction , gene
Caveolins have been identified as key components of caveolae, specialized cholesterol‐enriched raft domains visible as small flask‐shaped invaginations of the plasma membrane. In polarized MDCK cells caveolin‐1 and ‐2 are found together on basolateral caveolae whereas the apical membrane, where only caveolin‐1 is present, lacks caveolae. Expression of a caveolin mutant prevented the formation of the large caveolin‐1/‐2 hetero‐oligomeric complexes, and led to intracellular retention of caveolin‐2 and disappearance of caveolae from the basolateral membrane. Correspondingly, in MDCK cells over‐expressing caveolin‐2 the basolateral membrane exhibited an increased number of caveolae. These results indicate the involvement of caveolin‐2 in caveolar biogenesis.