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Hypertonic activation of phospholemman in solitary rat hepatocytes in primary culture
Author(s) -
Kirschner Udo,
Van Driessche Willy,
Werner Andreas,
Wehner Frank
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00115-7
Subject(s) - xenopus , tonicity , microbiology and biotechnology , chemistry , hepatocyte , gating , complementary dna , biophysics , conductance , primary culture , biology , biochemistry , gene , in vitro , physics , condensed matter physics
Under hypertonic conditions, solitary rat hepatocytes in primary culture shrink and subsequently exhibit a distinct regulatory volume increase (RVI). Reverse‐transcribed polymerase chain reaction and 5′ and 3′ RACE (rapid amplification of cDNA ends) techniques reveal that these cells express phospholemman (PLM). In whole‐cell recordings, the hypertonic activation of a channel is observed that resembles PLM with respect to unitary conductance (600–700 pS), gating pattern, and non‐selectivity for Na + over K + . In Xenopus oocytes expressing hepatocyte PLM, hypertonic stress induces a non‐selective cation conductance and noise analysis reveals the activation of a channel with a unitary conductance of approximately 700 pS. These results suggest a role of PLM in the RVI of rat hepatocytes.