Premium
Preliminary characterisation of mechanoresponsive regions of the c‐ fos promoter in bone cells
Author(s) -
Peake Matthew A.,
El Haj Alicia J.
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00108-x
Subject(s) - promoter activity , transfection , reporter gene , c fos , microbiology and biotechnology , bone cell , osteoblast , biology , chemistry , promoter , cell culture , gene , gene expression , genetics , in vitro
Preliminary analysis of the mechanisms involved in induction of stretch‐mediated transcriptional activity in the c‐ fos promoter of bone has been undertaken using a series of c‐ fos promoter–reporter constructs. UMR‐106 osteoblastic cells transfected with reporter constructs were subjected to cyclical physiological loading. The major determinants in the resulting transcriptional mechanoactivation are within the sequence between −356 and −151 which contains the serum response element and a consensus shear stress response element. Elements beyond this region also play a role as deletion of this region does not eliminate mechanoinduction. These results suggest that the mechanical induction of c‐ fos in osteoblastic bone cells is mediated by multiple response elements.