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Inhibition of the plant cytokinin transduction pathway by bacterial histidine kinase inhibitors in Catharanthus roseus cell cultures
Author(s) -
Papon Nicolas,
Clastre Marc,
Gantet Pascal,
Rideau Marc,
Chénieux Jean-Claude,
Crèche Joël
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00102-9
Subject(s) - catharanthus roseus , cytokinin , histidine kinase , biology , signal transduction , biochemistry , kinase , histidine , transduction (biophysics) , microbiology and biotechnology , enzyme , gene , auxin
We describe the isolation of two Catharanthus roseus cDNAs encoding proteins putatively involved in the final steps of a ‘histidine‐to‐aspartate’ phosphorelay in cytokinin (CK) signaling. The expression of one of these genes, CrRR1 , was specifically up‐regulated by CKs in C. roseus cell suspensions. We used this system as a biological model to test the activity of bacterial histidine kinase inhibitors. Our data demonstrate that these inhibitors are active on the CK transduction pathway and represent powerful chemical tools to study hormone signal transduction in plants. Moreover, these data suggest a strong conservation of functional features between prokaryotic and plant signaling pathways utilizing histidine kinases.