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Interaction of NF‐Y with the 3′‐flanking DNA sequence of the CCAAT box
Author(s) -
Sugiura Naoaki,
Takishima Kunio
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00071-1
Subject(s) - caat box , electrophoretic mobility shift assay , transcription factor , microbiology and biotechnology , dna , binding site , hmg box , transcription (linguistics) , dna binding protein , biology , genetics , gene expression , gene , promoter , linguistics , philosophy
NF‐Y, also referred to as CCAAT‐binding factor, is a major CCAAT‐binding transcription factor. The present study demonstrated that the 3′‐flanking region of the CCAAT box is involved in the formation of a stable NF‐Y·DNA complex. An electrophoretic mobility shift assay showed that the interaction of NF‐Y with DNA 15 bp downstream of the CCAAT box alters not only the affinity of NF‐Y for its binding site but also the electrophoretic mobility of the NF‐Y·DNA complex. This interaction is accompanied by a conformational change of NF‐Y as demonstrated by a change in the reactivity of an anti‐NF‐YA antibody to the NF‐Y·DNA complex.