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Bsp1p/Ypr171p is an adapter that directly links some synaptojanin family members to the cortical actin cytoskeleton in yeast
Author(s) -
Wicky Sidonie,
Frischmuth Sabine,
Singer-Krüger Birgit
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00067-x
Subject(s) - microbiology and biotechnology , actin cytoskeleton , phosphatidylinositol , cytoskeleton , actin binding protein , phosphatase , actin , biology , signal transducing adaptor protein , yeast , subcellular localization , pleckstrin homology domain , kinase , biochemistry , phosphorylation , cytoplasm , cell
In this study we identified a novel protein, Bsp1p, that interacts directly with two yeast synaptojanins, Sjl2p and Sjl3p, but not with Sjl1p. The interaction takes place via the Sac1/polyphosphoinositide phosphatase domain, whose conserved C‐terminal region is important for binding. Subcellular localization and genetic interactions revealed a function of Bsp1p in the cortical actin cytoskeleton. A fraction of Bsp1p was found to be membrane‐associated. Studies with mutants of phosphatidylinositol 4‐kinase, PIK1 , suggested that the interaction with membranes is facilitated by phosphoinositides. We propose that Bsp1p is an adapter that links Sjl2p and Sjl3p to the cortical actin cytoskeleton.