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Expression of syntaxin 1C, an alternative splice variant of HPC‐1/syntaxin 1A, is enhanced by phorbol‐ester stimulation in astroglioma: participation of the PKC signaling pathway 1
Author(s) -
Nakayama Takahiro,
Mikoshiba Katsuhiko,
Yamamori Tetsuo,
Akagawa Kimio
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00015-2
Subject(s) - syntaxin 3 , syntaxin , protein kinase c , phorbol , signal transduction , microbiology and biotechnology , forskolin , stimulation , downregulation and upregulation , biology , chemistry , gene , exocytosis , biochemistry , endocrinology , secretion
Syntaxin 1C is an alternative splice variant of HPC‐1/syntaxin 1A; the latter participates in neurotransmitter release and is assigned to the gene domain responsible for Williams’ syndrome (WS). It is expressed in the soluble fraction extracted from human astroglioma cell lines T98G and U87MG. Quantitative immunoblot and indirect immunofluorescence analyses revealed that the expression of syntaxin 1C was upregulated by phorbol 12‐myristate 13‐acetate (PMA), but not by forskolin. A protein kinase C (PKC) inhibitor suppressed this enhancement. These results suggest that syntaxin 1C expression is regulated via the PKC signal pathway. This is the first report of a signal transduction system that directly affects the expression of syntaxin protein.