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Unexpected catalytic site variation in phosphoprotein phosphatase homologues of cofactor‐dependent phosphoglycerate mutase
Author(s) -
Rigden Daniel J.
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(03)00014-0
Subject(s) - phosphoglycerate mutase , phosphoprotein , mutase , phosphatase , biochemistry , biology , cofactor , binding site , enzyme , phosphorylation , genetics , glycolysis
The cofactor‐dependent phosphoglycerate mutase (dPGM) superfamily contains, besides mutases, a variety of phosphatases, both broadly and narrowly substrate‐specific. Distant dPGM homologues, conspicuously abundant in microbial genomes, represent a challenge for functional annotation based on sequence comparison alone. Here we carry out sequence analysis and molecular modelling of two families of bacterial dPGM homologues, one the SixA phosphoprotein phosphatases, the other containing various proteins of no known molecular function. The models show how SixA proteins have adapted to phosphoprotein substrate and suggest that the second family may also encode phosphoprotein phosphatases. Unexpected variation in catalytic and substrate‐binding residues is observed in the models.