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In plants the alc gene expression system responds more rapidly following induction with acetaldehyde than with ethanol
Author(s) -
Junker Björn H.,
Chu Chengcai,
Sonnewald Uwe,
Willmitzer Lothar,
Fernie Alisdair R.
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03889-9
Subject(s) - acetaldehyde , inducer , transgene , aspergillus nidulans , activator (genetics) , reporter gene , gene expression , biochemistry , chemistry , ethanol , metabolite , gene , genetically modified crops , microbiology and biotechnology , enzyme inducer , enzyme , biology , mutant
It has recently been proposed that acetaldehyde is the physiological inducer of the alc gene system and hence indirectly the activator of the AlcA promoter in Aspergillus nidulans . Here we show that this chemical induces expression of a GUS (β‐ D ‐glucuronidase) reporter under the control of the alc gene system in transgenic potato tubers more rapidly than ethanol allowing tighter control of transgene expression. Furthermore by analysis of metabolite levels we demonstrate that the application of inducer has few effects on metabolism. We propose that this system is therefore ideal for the temporal regulation of important metabolic enzyme activities.