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Sphingosine‐1‐phosphate stimulates cortisol secretion
Author(s) -
Rábano Miriam,
Peña Ana,
Brizuela Leyre,
Marino Aida,
Macarulla José Marı́a,
Trueba Miguel,
Gómez-Muñoz Antonio
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03882-6
Subject(s) - sphingosine 1 phosphate , sphingosine , secretion , chemistry , phosphate , endocrinology , medicine , biochemistry , biology , receptor
We show here for the first time that sphingosine‐1‐phosphate (Sph‐1‐P) stimulates cortisol secretion in zona fasciculata cells of bovine adrenal glands. This effect was dependent upon protein kinase C (PKC) and extracellular Ca 2+ , and was inhibited by pertussis toxin. Sph‐1‐P activated phospholipase D (PLD) through a pertussis toxin‐sensitive mechanism, also involving extracellular Ca 2+ and PKC. Primary alcohols, which attenuate formation of phosphatidic acid (the product of PLD), and cell‐permeable ceramides, which inhibit PLD, blocked Sph‐1‐P‐induced cortisol secretion. In conclusion, Sph‐1‐P stimulates cortisol secretion through a mechanism involving Gi/o protein‐coupled receptors, extracellular Ca 2+ , PKC and PLD.