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Structural characterization of the O‐chain polysaccharide isolated from Bordetella avium ATCC 5086: variation on a theme
Author(s) -
Larocque Suzon,
Brisson Jean-Robert,
Thérisod Hélène,
Perry Malcolm B,
Caroff Martine
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03843-7
Subject(s) - polysaccharide , chemistry , lipopolysaccharide , hydrolysis , acid hydrolysis , lipid a , oligosaccharide , biochemistry , microbiology and biotechnology , biology , endocrinology
The O‐chain polysaccharide (O‐PS) of Bordetella avium was isolated from the lipopolysaccharide by mild acid hydrolysis to remove the lipid A, followed by hydrofluorolysis to remove the lipopolysaccharide core oligosaccharide leaving a residual O‐PS for structural analysis. High resolution 1 H and 13 C NMR and MALDI studies showed the O‐chain to be a polymer composed of 1,4‐linked 2‐acetamidino‐3‐[3‐hydroxybutanamido]‐2,3‐dideoxy‐β‐ D ‐glucopyranosyluronic acid residues.