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Identification of an Arabidopsis mitochondrial succinate–fumarate translocator
Author(s) -
Catoni Elisabetta,
Schwab Rebecca,
Hilpert Melanie,
Desimone Marcelo,
Schwacke Rainer,
Flügge Ulf-Ingo,
Schumacher Karin,
Frommer Wolf B.
Publication year - 2003
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03782-1
Subject(s) - arabidopsis , complementation , mutant , arabidopsis thaliana , biology , yeast , biochemistry , gene , microbiology and biotechnology
Complementation of a yeast acr1 mutant carrying a deletion of the succinate/fumarate carrier gene enabled functional identification of a mitochondrial succinate translocator from Arabidopsis thaliana (AtmSFC1). Thus complementation of yeast mutants is applicable also for identification and characterization of organellar transporters. Reverse transcription polymerase chain reaction and promoter‐GUS fusion showed expression of AtmSFC1 in 2 day old dark grown seedlings, which declined in cotyledons during further development, consistent with a role in export of fumarate for gluconeogenesis during lipid mobilization at early germination of Arabidopsis seeds. In mature plants, expression was found in developing and germinating pollen, suggesting a role in ethanolic fermentation.