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Stimulation of adhesion molecule expression by Helicobacter pylori and increased neutrophil adhesion to human umbilical vein endothelial cells
Author(s) -
Byrne M.F,
Corcoran P.A,
Atherton J.C,
Sheehan K.M,
Murray F.E,
Fitzgerald D.J,
Murphy J.F
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03728-6
Subject(s) - umbilical vein , cell adhesion molecule , helicobacter pylori , intercellular adhesion molecule 1 , adhesion , e selectin , human umbilical vein endothelial cell , microbiology and biotechnology , endothelial stem cell , cell adhesion , chemistry , immunology , biology , in vitro , medicine , biochemistry , organic chemistry
Helicobacter pylori upregulates endothelial adhesion molecules but the pattern is unclear. Human umbilical vein endothelial cells (HUVEC) were exposed to control medium or H. pylori 60190. Binding of monoclonal antibodies against P‐selectin, E‐selectin, vascular adhesion molecule‐1 (VCAM‐1) and intercellular adhesion molecule‐1 (ICAM‐1) was determined using enzyme‐linked immunosorbent assay. Binding of polymorphonuclear leukocytes to HUVEC was determined on cells exposed as above. After 6 h exposure to H. pylori , there were 30%, 124%, 167% and 100% increases in P‐selectin, E‐selectin, VCAM‐1 and ICAM‐1 levels and a 400% increase in polymorphonuclear leukocyte adhesion in HUVEC exposed to H. pylori . Effects of incubation for other intervals between 0 and 18 h are also described. H. pylori exerts some of its effects on gastric mucosa via gastric vasculature. This study gives insight into the pattern of H. pylori ‐associated endothelial adhesion molecule upregulation.