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Small interfering RNA and gene silencing in transgenic mice and rats
Author(s) -
Hasuwa Hidetoshi,
Kaseda Kazuhiro,
Einarsdottir Thorbjorg,
Okabe Masaru
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03680-3
Subject(s) - rna interference , gene silencing , small interfering rna , transgene , biology , microbiology and biotechnology , trans acting sirna , rna silencing , gene , embryonic stem cell , rna , genetics
After short duplexes of synthetic 21–23 nt RNAs (siRNA) were reported to be effective in silencing specific genes, a vector‐based approach for siRNAs was demonstrated in mammalian cultured cell lines. However, the effect of RNA interference (RNAi) on various differentiated cells in live animals remains unknown. In this report, we demonstrate that transgenically supplied siRNA can silence ubiquitously expressed enhanced green fluorescent protein in every part of the mouse and rat body. These results suggest that transgenic RNAi could function as an alternative method of gene silencing by applying homologous recombination to embryonic stem (ES) cells, and should be successful even in species where ES cell lines remain unestablished.