Premium
Involvement of in vivo induced cheY‐4 gene of Vibrio cholerae in motility, early adherence to intestinal epithelial cells and regulation of virulence factors
Author(s) -
Banerjee Rajat,
Das Soumita,
Mukhopadhyay Kanchan,
Nag Sanjay,
Chakrabortty Amit,
Chaudhuri Keya
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03678-5
Subject(s) - vibrio cholerae , virulence , motility , microbiology and biotechnology , in vivo , vibrionaceae , virulence factor , gene , intestinal epithelium , biology , biofilm , epithelium , chemistry , bacteria , biochemistry , genetics
Using a global transcription profile approach cheY‐4 of Vibrio cholerae was identified as an in vivo induced gene. In the present study, duplication of the gene in the chromosome resulted in increased motility, increased chemotactic response towards isolated intestinal mucus layer and stronger adhesion to human intestinal epithelial cell line at an early phase of infection compared to wild type and a null mutant strain. In contrast to the cheY‐4 null mutant, duplication of cheY‐4 gene resulted in increased expression of ctxAB and tcpA , the two major virulence genes of V. cholerae .