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Maleimides stimulate oxygen reduction in illuminated thylakoids
Author(s) -
Ivanov Boris,
Ignat'ev Alexander,
Khorobrykh Sergey
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03673-6
Subject(s) - thylakoid , chemistry , electron transport chain , oxygen , superoxide , superoxide dismutase , catalase , biophysics , dithiothreitol , photochemistry , photosynthesis , biochemistry , stimulation , dcmu , radical , photosystem ii , chloroplast , biology , enzyme , organic chemistry , neuroscience , gene
N ‐ethylmaleimide (NEM) and N , N ′‐(1,4‐phenylene)dimaleimide (PDM) were discovered to stimulate light‐induced oxygen uptake in isolated thylakoids, and PDM provided the same stimulation at one order less concentrations. Oxygen uptake rate increased promptly after NEM or PDM addition to thylakoids. The inhibitors of photosynthetic electron transport as well as catalase decreased this rate close to zero, whereas ascorbate increased it almost three‐fold. Dithiothreitol suppressed oxygen uptake stimulated by NEM. NEM stimulated light‐induced reduction of cytochrome c , and this stimulation was suppressed by superoxide dismutase. It was concluded that NEM and PDM being reduced can effectively reduce molecules O 2 producing superoxide radicals.