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Enhancement of protein expression in insect cells by a lobster tropomyosin cDNA leader sequence
Author(s) -
Sano Ken-Ichi,
Maeda Kayo,
Oki Manami,
Maéda Yuichiro
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03659-1
Subject(s) - complementary dna , polyhedrin , tropomyosin , biology , microbiology and biotechnology , sequence (biology) , untranslated region , gene , expression vector , gene expression , genetics , messenger rna , recombinant dna , spodoptera , actin
We describe a cis element that dramatically increases the expression levels of exogenous genes in baculovirus‐infected insect cells. This 21 bp sequence element is derived from a 5′ untranslated leader sequence of a lobster tropomyosin cDNA (L21). By using a transfer vector carrying L21, the expression levels of tropomyosin and luciferase were 20‐ and seven‐fold higher with L21 than without L21, respectively. L21 has both the Kozak sequence and the A‐rich sequence found in the polyhedrin leader sequence. We assume that both sequence elements are essential for the enhancement of protein expression in the baculovirus‐based expression system.