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Identification of a novel RNA silencing suppressor, NSs protein of Tomato spotted wilt virus
Author(s) -
Takeda Atsushi,
Sugiyama Kazuhiko,
Nagano Hideaki,
Mori Masashi,
Kaido Masanori,
Mise Kazuyuki,
Tsuda Shinya,
Okuno Tetsuro
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03632-3
Subject(s) - rna silencing , gene silencing , biology , rna , rna interference , rna induced silencing complex , transgene , suppressor , rna induced transcriptional silencing , trans acting sirna , gene , small interfering rna , antisense rna , sense (electronics) , virology , microbiology and biotechnology , genetics , chemistry
RNA silencing or post‐transcriptional gene silencing (PTGS) in plants is known as a defense system against virus infection. Several plant viruses have been shown to encode an RNA silencing suppressor. Using a green fluorescent protein‐based transient suppression assay, we show that NSs protein of Tomato spotted wilt virus (TSWV) has RNA silencing suppressor activity. TSWV NSs protein suppressed sense transgene‐induced PTGS but did not suppress inverted repeat transgene‐induced PTGS. TSWV NSs protein is the first RNA silencing suppressor identified in negative‐strand RNA viruses.