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A recessive mutant cell line with a constitutive IκB kinase activity
Author(s) -
Chinawait Netnaphis,
Miura Hideyasu,
Yamamoto Naoki,
Yamaoka Shoji
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03618-9
Subject(s) - mutant , iκb kinase , somatic cell , cell culture , kinase , microbiology and biotechnology , biology , phenotype , nfkb1 , mutation , signal transduction , nf κb , genetics , gene , transcription factor
To search for negative regulatory components of the NF‐κB activation pathways, we mutagenized Rat‐1 fibroblasts and established a stable mutant cell line with a constitutive NF‐κB activity. This mutant cell line, designated as TK26, showed permanently elevated I kappa B kinase (IKK) activity and a genetically recessive phenotype revealed by somatic cell hybridization between TK26 and Rat‐1. Our results suggested that lack of a negative regulation of IKK could lead to permanent NF‐κB activation. The TK26 cell line will be useful to genetically identify a component necessary for keeping the IKK complex under an inactive form in resting cells.