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Effect of annexin I on insulin secretion through surface binding sites in rat pancreatic islets
Author(s) -
Hong Shin-Hee,
Won Jong Hak,
Yoo Seung-Ah,
Auh Chung-Kyoon,
Park Young Min
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03613-x
Subject(s) - pancreatic islets , medicine , endocrinology , extracellular , insulin , secretion , islet , annexin , chemistry , annexin a1 , annexin a2 , biology , microbiology and biotechnology , cell , biochemistry
This study investigates the effect of extracellular annexin I (Anx I) on regulating insulin secretion in isolated rat pancreatic islets. Results show that Anx I stimulates insulin release in pancreatic islets regardless of the presence or absence of extracellular Ca 2+ . In particular, confocal microscopy shows that Anx I binds to the surface of islet cells in the absence of extracellular Ca 2+ . However, insulin secretion through Anx I significantly decreases in trypsin‐treated islets. Likewise, there is minimal binding of Anx I to the surface of trypsin‐treated islets. Anti‐Anx I polyclonal antibody also inhibits the stimulating effect of Anx I on insulin secretion. These results indicate that Anx I is capable of binding to the cell surface receptor, in order to regulate the stimulation of insulin release in rat pancreatic islets.