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Activity of the A3 adenosine receptor gene promoter in transgenic mice: characterization of previously unidentified sites of expression
Author(s) -
Yaar Ron,
Lamperti Edward D,
Toselli Paul A,
Ravid Katya
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03612-8
Subject(s) - transgene , reporter gene , microbiology and biotechnology , gene expression , biology , genetically modified mouse , gene , adenosine , adenosine receptor , adenosine a3 receptor , adenosine a2b receptor , promoter , receptor , biochemistry , agonist
Sites of A3 adenosine receptor gene expression have not been fully explored nor has this gene's promoter activity been confirmed in vivo. Transgenic mice were generated in which 2.3 kb upstream of the transcriptional start site of the mouse A3 adenosine receptor was coupled to a β‐galactosidase reporter gene. Selective transgene expression was detected in testis and brain as well as at other sites in which A3 adenosine receptor message has not been previously reported, including retinal ganglion cells and smooth muscle cells of the cerebrospinal vasculature. Our study suggests that this promoter may be useful in the selective targeting of gene expression to specific tissues.