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An enhancer complex confers both high‐level and cell‐specific expression of the human type X collagen gene
Author(s) -
Chambers David,
Young David A.,
Howard Charles,
Thomas J.Terrig,
Boam David S.,
Grant Michael E.,
Wallis Gillian A.,
Boot-Handford Raymond P.
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03606-2
Subject(s) - enhancer , reporter gene , microbiology and biotechnology , type ii collagen , transfection , endochondral ossification , chemistry , regulation of gene expression , cell growth , gene expression , transcription (linguistics) , biology , cell type , gene , cell , cartilage , biochemistry , anatomy , linguistics , philosophy
Type X collagen expression is restricted to hypertrophic chondrocytes in the endochondral growth plate. Transient transfection of reporter constructs containing the human collagen X promoter into primary growth plate chondrocytes identified a cis ‐acting positive regulatory DNA element(s) that has cell‐specific enhancer properties and binds a nuclear protein expressed specifically in growth plate chondrocytes. Functional disruption of this region results in a significant reduction in the activation of reporter gene transcription. The identified enhancer is a major element controlling both high‐level and cell‐specific expression of type X collagen gene.