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Human pancreas protein 2 (PAN2) has a retinal reductase activity and is ubiquitously expressed in human tissues
Author(s) -
Belyaeva Olga V,
Kedishvili Natalia Y
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03588-3
Subject(s) - nad+ kinase , reductase , biochemistry , aldo keto reductase , retinaldehyde , enzyme , biology , 7 dehydrocholesterol reductase , microbiology and biotechnology , chemistry , gene , retinoic acid
Human gene for pancreas protein 2 (PAN2) is a novel member of the short‐chain dehydrogenase/reductase gene superfamily. The properties of PAN2 protein have not yet been characterized. We present the first evidence that human PAN2 is a ubiquitously expressed microsomal enzyme that recognizes retinoids but not steroids as substrates with the apparent K m values between 0.08 μM and 0.4 μM. PAN2 is ∼4‐fold more efficient in the reductive than in the oxidative direction. The apparent K m values for NADP + and NADPH are 0.65 μM and 0.32 μM versus 1200 μM and 1060 μM for NAD + and NADH, respectively. Kinetic constants and expression pattern of PAN2 suggest that it is likely to function as a reductase in vivo and might contribute to the reduction of retinaldehyde to retinol in most human tissues.