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Pyrococcus furiosus ferredoxin is a functional dimer
Author(s) -
Hasan M.N,
Hagedoorn P.L,
Hagen W.R
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03554-8
Subject(s) - pyrococcus furiosus , ferredoxin , dimer , chemistry , ionic strength , crystallography , ionic bonding , monomer , electron paramagnetic resonance , photochemistry , biochemistry , ion , nuclear magnetic resonance , enzyme , organic chemistry , archaea , physics , aqueous solution , gene , polymer
Pyrococcus furiosus ferredoxin is subject to a monomer/dimer equilibrium as a function of ionic strength. At physiological ionic strength, approximately 0.35 M NaCl, the protein is very predominantly homodimer. The monomeric form exhibits impaired electron transfer on glassy carbon; it also has a decreased S =3/2 over S =1/2 ratio as shown by electron paramagnetic resonance spectroscopy. Even following sterilization at 121°C the dimer is stable in denaturing gel electrophoresis.