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Anti‐HIV‐1 property of trichosanthin correlates with its ribosome inactivating activity
Author(s) -
Wang Jian-Hua,
Nie Hui-Ling,
Tam Siu-Cheung,
Huang Hai,
Zheng Yong-Tang
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03539-1
Subject(s) - trichosanthin , ribosome inactivating protein , human immunodeficiency virus (hiv) , ribosome , chemistry , property (philosophy) , biology , biochemistry , biophysics , virology , philosophy , rna , gene , epistemology
Trichosanthin (TCS) is a type I ribosome inactivating (RI) protein possessing anti‐tumor and antiviral activity, including human immunodeficiency virus (HIV). The mechanism of these actions is not entirely clear, but is generally attributed to its RI property. In order to study the relationship between the anti‐HIV‐1 activity of TCS and its RI activity, three TCS mutants with different RI activities were constructed by using site‐directed mutagenesis. The anti‐HIV‐1 activities of the three mutants were tested in vitro. Results showed that two TCS mutants, namely TCS M(120–123) , TCS E160A/E189A , with the greatest decrease in RI activity, lost almost all of the anti‐HIV activity and cytopathic effect. Another mutant TCS R122G , which exhibited a 160‐fold decrease in RI activity, retained some anti‐HIV activity. The results from this study suggested that RI activity of TCS may have significant contribution to its anti‐HIV‐1 property.