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Gating of the expressed Ca v 3.1 calcium channel
Author(s) -
Lacinová L’,
Klugbauer N,
Hofmann F
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03509-3
Subject(s) - depolarization , gating , membrane potential , chemistry , biophysics , ion channel , charge (physics) , voltage gated ion channel , physics , biology , biochemistry , receptor , quantum mechanics
Intramembrane charge movement originating from Ca v 3.1 (T‐type) channel expressed in HEK 293 cells was investigated. Ion current was blocked by 1 mM La 3+ . Charge movement was detectable for depolarizations above ∼−70 mV and saturated above +60 mV. The voltage dependence of charge movement followed a single Boltzmann function with half‐maximal activation voltage +12.9 mV and +12.3 mV and with slopes of 22.4 mV and 18.1 mV for the ON‐ and OFF‐charge movement, respectively. Inactivation of I Ca by prolonged depolarization pulse did not immobilize intramembrane charge movement in the Ca v 3.1 channel.