Premium
Zonal distribution of glycogen synthesis in isolated rat hepatocytes
Author(s) -
Fernández-Novell Josep M,
López-Iglesias Carmen,
Ferrer Juan C,
Guinovart Joan J
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03506-8
Subject(s) - glycogen , incubation , hepatocyte , glycogen synthase , glycogen branching enzyme , biochemistry , incubation period , chemistry , biology , medicine , endocrinology , in vitro
Incubation of hepatocytes isolated from fasted rats with [ 14 C]glucose for short periods of time showed that the initial stages of glycogen synthesis occur near the plasma membrane. Incubation with [ 14 C]glucose followed by cold glucose demonstrated that glycogen synthesis is always active at the hepatocyte periphery and that previously synthesised glycogen moves towards the centre of the cell, while its place is filled by newly synthesised molecules. However, the reverse experiment, incubation with cold glucose before addition of [ 14 C]glucose, showed that, as glycogen synthesis progresses, it also becomes gradually active in more internal sites of the hepatocyte. These results indicate a spatial order in the synthesis of hepatic glycogen.