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PIAS3 induces SUMO‐1 modification and transcriptional repression of IRF‐1
Author(s) -
Nakagawa Koji,
Yokosawa Hideyoshi
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03486-5
Subject(s) - repressor , ubiquitin ligase , sumo protein , psychological repression , activator (genetics) , ubiquitin conjugating enzyme , ubiquitin , irf1 , transcriptional regulation , biology , microbiology and biotechnology , transcription factor , chemistry , biochemistry , gene expression , gene
Interferon regulatory factor‐1 (IRF‐1) is a transcriptional activator that is involved in interferon response, regulation of cell growth and oncogenesis. To try to identify the molecules that regulate the function of IRF‐1, we performed yeast two‐hybrid screening and isolated protein inhibitor of activated STAT3 (PIAS3) as an IRF‐1‐binding protein. This protein was also found to bind with small ubiquitin‐related modifier‐1 (SUMO‐1) and ubiquitin‐conjugating enzyme 9, an E2 in the SUMO‐1‐conjugating system. Co‐expression of PIAS3 induced SUMO‐1 modification of IRF‐1 in a RING finger domain‐dependent manner and also repressed transcriptional activity of IRF‐1. Thus, PIAS3 functions as a SUMO‐1 ligase for IRF‐1 and also as a repressor of IRF‐1 transcriptional activity.