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Monosialyl‐Gb5 organized with cSrc and FAK in GEM of human breast carcinoma MCF‐7 cells defines their invasive properties
Author(s) -
Steelant Wim F,
Kawakami Yasushi,
Ito Akihiro,
Handa Kazuko,
Bruyneel Erik A,
Mareel Marc,
Hakomori Senitiroh
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03484-1
Subject(s) - mcf 7 , motility , cancer research , human breast , malignancy , breast carcinoma , chemistry , pathology , biology , microbiology and biotechnology , cancer cell , breast cancer , cancer , medicine
Two human mammary carcinoma cell variants, MCF‐7/AZ and MCF‐7/6, show the same composition in their glycosphingolipid‐enriched microdomain (GEM) with regard to globo‐series structures Gb3, Gb4, Gb5, monosialyl‐Gb5, GM2, and cSrc and FAK. Both variants are non‐invasive into collagen gel layer, and showed similar motility in wound migration assay. Whereas invasiveness and motility of MCF‐7/AZ cells were enhanced greatly by treatment with mAb RM1 directed to monosialyl‐Gb5, the same RM1 treatment had no effect on MCF‐7/6. cSrc and FAK of MCF‐7/AZ, but not MCF‐7/6, were activated by RM1 treatment. Thus, malignancy of MCF‐7 is highly dependent on monosialyl‐Gb5, and its activation of cSrc and FAK in GEM.