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Testosterone inhibits tumor necrosis factor‐α‐induced vascular cell adhesion molecule‐1 expression in human aortic endothelial cells
Author(s) -
Hatakeyama Haruhiko,
Nishizawa Makoto,
Nakagawa Atsushi,
Nakano Shigeru,
Kigoshi Toshikazu,
Uchida Kenzo
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03440-3
Subject(s) - cell adhesion molecule , tumor necrosis factor alpha , microbiology and biotechnology , chemistry , adhesion , necrosis , cell adhesion , tumor necrosis factor α , cancer research , cell , endocrinology , medicine , biology , biochemistry , organic chemistry
We investigated the effect of testosterone (T) on tumor necrosis factor‐α (TNF‐α)‐induced expression of vascular cell adhesion molecule‐1 (VCAM‐1) in human aortic endothelial cells. Incubation of these cells with T resulted in a dose‐dependent reduction in the expression, with this reduction completely abolished by a selective androgen receptor blocker. Electrophoretic mobility shift assay demonstrated that T inhibited TNF‐α‐induced activation of the transcriptional nuclear factor‐κB, which is critical for the inducible expression of VCAM‐1, probably through the suppression of the nuclear translocation. Our results may suggest an inhibitory effect of T on atherogenesis, providing a novel insight into the consideration of the pathogenesis of atherosclerosis.