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V‐1, a catecholamine biosynthesis regulatory protein, positively controls catecholamine secretion in PC12D cells
Author(s) -
Yamakuni Tohru,
Yamamoto Toshifumi,
Ishida Yukisato,
Yamamoto Hideko,
Song Si-Young,
Adachi Eijiro,
Hiwatashi Yusuke,
Ohizumi Yasushi
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03431-2
Subject(s) - catecholamine , secretion , biosynthesis , medicine , endocrinology , chemistry , biology , biochemistry , gene
Stably transfected PC12D cell lines overexpressing a catecholamine biosynthesis regulatory protein, V‐1, were used to examine the functional role of V‐1 in catecholamine secretion. High K + ‐induced dopamine secretion in V‐1 overexpressing clones was shown to be markedly potentiated compared with control clones carried with a vector alone. As assayed intracellular calcium concentration ([Ca 2+ ] i ) using fura‐PE3, V‐1 overexpression was observed to enhance high K + ‐elicited [Ca 2+ ] i elevation. Electron microscopic analysis revealed an increase in dense‐cored vesicle formation by V‐1 overexpression. These results suggest that the enhancement of high K + ‐induced dopamine secretion by V‐1 overexpression results from the potentiation of high K + ‐induced [Ca 2+ ] i elevation and the increase in the number of dense‐cored vesicles.