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A comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis
Author(s) -
Hunt Alan N,
Clark Graeme T,
Neale James R,
Postle Anthony D
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03429-4
Subject(s) - phosphatidylcholine , diacylglycerol kinase , phosphatidylinositol , polyunsaturated fatty acid , phospholipid , biochemistry , chemistry , choline , fatty acid , enzyme , signal transduction , membrane , protein kinase c
Deuterated choline‐ d 9 labelling of IMR‐32 cells enabled comparison of the molecular specificities of whole cell and endonuclear phosphatidylcholine synthesis after 96 h polyunsaturated fatty acid supplementation. Surprisingly, while cell phosphatidylcholine synthesis and remodelling reflected a pattern of polyunsaturated fatty acid accretion, the saturated endonuclear phosphatidylcholine pool was only transiently labelled with polyunsaturates. Periodic endonuclear accumulations of the lipid second messenger diacylglycerol, mobilised from unsaturated phosphatidylinositol or saturated phosphatidylcholine, accompany cell proliferation. Non‐specific incorporation into endonuclear phosphatidylcholine and selective removal or remodelling of unsaturated molecular species may form part of a single ‘off switch’ recycling all endonuclear diacylglycerol accumulations.