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Embryonic brain expression analysis of lysophospholipid receptor genes suggests roles for s1p 1 in neurogenesis and s1p 1–3 in angiogenesis
Author(s) -
McGiffert Christine,
Contos James J.A,
Friedman Beth,
Chun Jerold
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03404-x
Subject(s) - neurogenesis , biology , angiogenesis , lysophosphatidic acid , in situ hybridization , northern blot , sphingosine 1 phosphate , microbiology and biotechnology , lateral ventricles , embryonic stem cell , receptor , gene expression , sphingosine , anatomy , gene , genetics
In a comparison of embryonic brain expression patterns of lysophosphatidic acid and sphingosine 1‐phosphate receptor genes ( lpa 1–3 and s1p 1–5 , respectively), transcripts detected by Northern blot were subsequently localized using in situ hybridization. We found striking s1p 1 expression adjacent to several ventricles. Near the lateral ventricle, s1p 1 expression was temporally and spatially coincident with neurogenesis and overlapped with lpa 1 in the neocortical area. We also observed a widespread diffuse pattern for lpa 2–3 and a scattered punctate pattern for s1p 1–3 . The punctate pattern colocalized with vascular endothelial markers. Together, these results suggest that s1p 1 influences neurogenesis and s1p 1–3 influence angiogenesis in the developing brain.