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The short N‐terminus is required for functional expression of the virus‐encoded miniature K + channel Kcv
Author(s) -
Moroni Anna,
Viscomi Carlo,
Sangiorgio Vanessa,
Pagliuca Cinzia,
Meckel Tobias,
Horvath Ferenc,
Gazzarrini Sabrina,
Valbuzzi Paola,
Van Etten James L,
DiFrancesco Dario,
Thiel Gerhard
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03397-5
Subject(s) - hek 293 cells , transfection , microbiology and biotechnology , xenopus , c terminus , cytoplasm , chemistry , biology , amino acid , biochemistry , gene
Kcv (K + Chlorella virus) is a miniature virus‐encoded K + channel. Its predicted membrane–pore–membrane structure lacks a cytoplasmic C‐terminus and it has a short 12 amino acid (aa) cytoplasmic N‐terminus. Kcv forms a functional channel when expressed in human HEK 293 cells. Deletion of the 14 N‐terminal aa results in no apparent differences in the subcellular location and expression level of the Kcv protein. However, the truncated protein does not induce a measurable current in transfected HEK 293 cells or Xenopus oocytes. We conclude that the N‐terminus controls functional properties of the Kcv channel, but does not influence protein expression.