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β 1 /β 2 /β 3 ‐adrenoceptor knockout mice are obese and cold‐sensitive but have normal lipolytic responses to fasting
Author(s) -
Jimenez Maria,
Léger Bertrand,
Canola Kriss,
Lehr Lorenz,
Arboit Patrizia,
Seydoux Josiane,
Russell Aaron P,
Giacobino Jean-Paul,
Muzzin Patrick,
Preitner Frédéric
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03387-2
Subject(s) - lipolysis , endocrinology , medicine , thermogenesis , basal (medicine) , beta (programming language) , knockout mouse , thermogenin , adrenergic receptor , chemistry , brown adipose tissue , beta 3 adrenergic receptor , obesity , adipose tissue , biology , receptor , insulin , computer science , programming language
Catecholamines are viewed as major stimulants of diet‐ and cold‐induced thermogenesis and of fasting‐induced lipolysis, through the β‐adrenoceptors (β 1 /β 2 /β 3 ). To test this hypothesis, we generated β 1 /β 2 /β 3 ‐adrenoceptor triple knockout (TKO) mice and compared them to wild type animals. TKO mice exhibited normophagic obesity and cold‐intolerance. Their brown fat had impaired morphology and lacked responses to cold of uncoupling protein‐1 expression. In contrast, TKO mice had higher circulating levels of free fatty acids and glycerol at basal and fasted states, suggesting enhanced lipolysis. Hence, β‐adrenergic signalling is essential for the resistance to obesity and cold, but not for the lipolytic response to fasting.