Premium
Regulatory expression of MDP77 protein in the skeletal and cardiac muscles
Author(s) -
Fujimori Kazuhiro E,
Uyeda Atsuko,
Taguchi Takahisa
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03386-0
Subject(s) - sarcomere , skeletal muscle , cardiac muscle , in vivo , polyclonal antibodies , myocyte , microbiology and biotechnology , biology , gene expression , in vitro , function (biology) , anatomy , gene , endocrinology , antibody , biochemistry , genetics
The mdp77 gene was first cloned from the cDNA library of denervated chick muscles, while its role(s) in vivo was unknown. In the present study, using specific polyclonal antibodies against MDP77, we show that MDP77 was expressed specifically in the skeletal and cardiac muscle, and confirm its presence in the cytoplasm of the extrafusal muscle fibers. In mature muscles, MDP77 immunoreactivity was observed in a repetitive manner along the sarcomere. The onset of MDP77 expression occurred just after myotube formation both in vivo and in vitro. Furthermore, MDP77 was enriched in the intrafusal muscle fibers. Our findings suggest that MDP77 plays an important role(s) in the differentiation, maturation and function of both the skeletal and cardiac muscles.