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WARTS tumor suppressor is phosphorylated by Cdc2/cyclin B at spindle poles during mitosis
Author(s) -
Morisaki Tetsuro,
Hirota Toru,
Iida Shin-ichi,
Marumoto Tomotoshi,
Hara Toshihiro,
Nishiyama Yasuyuki,
Kawasuzi Michio,
Hiraoka Takehisa,
Mimori Tatsuyuki,
Araki Norie,
Izawa Ichiro,
Inagaki Masaki,
Saya Hideyuki
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03360-4
Subject(s) - prometaphase , cyclin b , microbiology and biotechnology , cyclin a , mitosis , aurora b kinase , cyclin a2 , cyclin b1 , cyclin d , biology , spindle checkpoint , polo like kinase , cyclin dependent kinase 1 , spindle apparatus , cancer research , cyclin , chemistry , cell cycle , phosphorylation , anaphase , protein kinase a , genetics , cyclin dependent kinase 2 , cell division , cancer , cell
Identification of physiological substrates for Cdc2/cyclin B is crucial for understanding the functional link between mitotic events and Cdc2/cyclin B activation. A human homologue of the Drosophila warts tumor suppressor, termed WARTS, is a serine/threonine kinase and a dynamic component of the mitotic apparatus. We have found that Cdc2/cyclin B forms a complex with a fraction of WARTS in the centrosome and phosphorylates the Ser613 site of WARTS during mitosis. Immunocytochemical analysis has shown that the S613‐phosphorylated WARTS appears in the spindle poles at prometaphase and disappears at telophase. Our findings suggest that Cdc/cyclin B regulates functions of WARTS on the mitotic apparatus.