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Alternative splicing of the rat Ca v 3.3 T‐type calcium channel gene produces variants with distinct functional properties 1
Author(s) -
Murbartián Janet,
Arias Juan Manuel,
Lee Jung-Ha,
Gomora Juan Carlos,
Perez-Reyes Edward
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03341-0
Subject(s) - alternative splicing , exon , rna splicing , gene , splice , biology , genetics , gene expression , microbiology and biotechnology , rna
Molecular diversity in T‐type Ca 2+ channels is produced by expression of three genes, and alternative splicing of those genes. Prompted by differences noted between rat and human Ca v 3.3 sequences, we searched for splice variants. We cloned six variants, which are produced by splicing at exon 33 and exon 34. Expression of the variants differed between brain regions. The electrophysiological properties of the variants displayed similar voltage‐dependent gating, but differed in their kinetic properties. The functional impact of splicing was inter‐related, suggesting an interaction. We conclude that alternative splicing of the Ca v 3.3 gene produces channels with distinct properties.