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Transcriptional activator, AoXlnR, mediates cellulose‐inductive expression of the xylanolytic and cellulolytic genes in Aspergillus oryzae
Author(s) -
Marui Junichiro,
Kitamoto Noriyuki,
Kato Masashi,
Kobayashi Tetsuo,
Tsukagoshi Norihiro
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03328-8
Subject(s) - aspergillus oryzae , gene , xylanase , biology , gene expression , xylan , aspergillus , cellulase , activator (genetics) , microbiology and biotechnology , cellulose , biochemistry , enzyme
AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1 , in Aspergillus oryzae . To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain was analyzed by Northern blotting. AoXlnR mediated expression of at least four xylanolytic genes and four cellulolytic genes when induced by xylan and D ‐xylose. Moreover, AoXlnR was newly found to mediate the cellulose‐inductive expression of the xylanolytic genes as well as the cellulolytic genes.