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Increased stability of human growth hormone with reduced lactogenic potency
Author(s) -
Schulga Alexey A,
Makarov Alexander A,
Levichkin Iliya V,
Belousova Yuliya V,
Lobachov Vladimir M,
Protasevich Irina I,
Pace C.Nick,
Kirpichnikov Mikhail P
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03325-2
Subject(s) - potency , prolactin , hormone , stimulation , lactation , chemistry , endocrinology , medicine , escherichia coli , biology , biochemistry , microbiology and biotechnology , in vitro , genetics , pregnancy , gene
Human growth hormone (hGH), whose main function is the somatic growth stimulation, induces diverse effects including lactation. We examined the possibility of hGH stabilization by elimination of its lactogenic activity. Chimeric GHs were constructed by replacement of different segments of hGH with sequences derived from non‐lactogenic porcine GH. As was observed in the rat Nb2‐11C lymphoma cell test, lactogenic activity of some chimeric hormones was seriously destroyed. This kind of hormones displayed the substantial increase in thermal and guanidine hydrochloride stability. The more stable hGH variants were found to be more soluble in Escherichia coli cells.