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Effect of cholesterol on the interaction of seminal plasma protein, PDC‐109 with phosphatidylcholine membranes
Author(s) -
Swamy Musti J,
Marsh D,
Anbazhagan V,
Ramakrishnan M
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03316-1
Subject(s) - phosphatidylcholine , chemistry , membrane , cholesterol , biophysics , microbiology and biotechnology , biochemistry , phospholipid , biology
Binding of PDC‐109, the major protein of the bovine seminal plasma, to sperm plasma membrane results in an efflux of cholesterol and choline phospholipids, a necessary event before capacitation can occur. The selectivity of PDC‐109 for different spin‐labelled phospholipids and sterol probes in dimyristoylphosphatidylcholine (DMPC) host matrix has been characterized earlier by EPR spectroscopy [Ramakrishnan, M., Anbazhagan, V., Pratap, T.V., Marsh, D. and Swamy, M.J. (2001) Biophys. J. 81, 2215–2225]. In this report the effect of cholesterol on the interaction of PDC‐109 with DMPC membranes has been investigated by spin‐label EPR spectroscopy. The results indicate that the presence of cholesterol leads to an increased association of different phospholipid as well as sterol probes, thus modulating the interaction of PDC‐109 with phospholipid membranes.