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Fluorescence of native and carotenoid‐depleted LH2 from Chromatium minutissimum , originating from simultaneous two‐photon absorption in the spectral range of the presumed (optically ‘dark’) S 1 state of carotenoids
Author(s) -
Krikunova M,
Kummrow A,
Voigt B,
Rini M,
Lokstein H,
Moskalenko A,
Scheer H,
Razjivin A,
Leupold D
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03315-x
Subject(s) - bacteriochlorophyll , fluorescence , chromatium , absorption (acoustics) , excited state , photochemistry , chemistry , physics , analytical chemistry (journal) , atomic physics , photosynthesis , optics , biochemistry , chromatography
Native and carotenoid‐depleted peripheral purple bacterial light‐harvesting complex (LH2) were investigated by simultaneous two‐photon excited (between 1300–1500 nm) fluorescence (TPF). TPF results from direct bacteriochlorophyll excitation in both samples. The spectral position of the 2A g − state of rhodopsin is indicated by a diminuition of the bacteriochlorophyll TPF in native LH2. In conclusion, comparison to carotenoid‐depleted samples is a conditio sine qua non for unambiguous interpretation of similar experiments.