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Ectodomain shedding of furin: kinetics and role of the cysteine‐rich region
Author(s) -
Denault Jean-Bernard,
Bissonnette Lyne,
Longpré Jean-Michel,
Charest Gabriel,
Lavigne Pierre,
Leduc Richard
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03249-0
Subject(s) - ectodomain , furin , kinetics , cysteine , chemistry , microbiology and biotechnology , biochemistry , biophysics , biology , receptor , enzyme , physics , quantum mechanics
Furin, a member of the subtilisin‐like pro‐protein convertase family, is a type I membrane protein that undergoes ectodomain shedding. Metabolic labeling of cells stably expressing furin demonstrated that the shed form of furin is detected after 30 min. Moreover, sequence analysis revealed that specific residues of the cysteine‐rich region of furin aligned with those of tumor necrosis factor receptor, which is also shed. Introduction within furin's cysteine‐rich region of mutations that impair TNFR1 shedding also abolished furin shedding. Our results show that shedding of furin occurs rapidly and further suggest that specific cysteine residues may impart a conformation to the enzyme, thereby affecting its susceptibility to proteolysis.