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A selective effect of Mpl ligand on mRNA stabilization during megakaryocyte differentiation
Author(s) -
Kaluzhny Yulia,
Hechler Beatrice,
Lu Jun,
Nguyen Hao G,
Cataldo Leah M,
Ravid Katya
Publication year - 2002
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/s0014-5793(02)03230-1
Subject(s) - megakaryocyte , ligand (biochemistry) , chemistry , messenger rna , receptor , microbiology and biotechnology , platelet , platelet factor 4 , untranslated region , biology , biochemistry , haematopoiesis , immunology , gene , stem cell
Megakaryocytes, the platelet precursors, are induced to differentiate in response to Mpl ligand. Here we report that stability of the megakaryocyte‐specific platelet factor 4 (PF4) mRNA is substantially augmented in the presence of Mpl ligand. This stabilization requires protein synthesis, but the 3′‐untranslated region of PF4 mRNA is not sufficient for granting the effect. This cytokine also significantly or mildly stabilizes Mpl receptor or GAPDH mRNAs, respectively, in contrast to a previously reported lack of effect on P2Y 1 receptor mRNA. Our study is the first to suggest that Mpl ligand‐induced lineage specification is also determined by message stabilization.